Purpose

The researchers will investigate if modified T-cells from a patients own system can be utilized to find and destroy metastatic melanoma tumor and thus improve patient outcomes.

Condition

Eligibility

Eligible Ages
Between 18 Years and 66 Years
Eligible Genders
All
Accepts Healthy Volunteers
No

Inclusion Criteria

  • Metastatic, surgically unresectable melanoma or newly diagnosed melanoma of any stage, where the patient is unable to receive or complete standard therapy
  • Life expectancy of at least 12 weeks.
  • Eastern Cooperative Oncology Group (ECOG) performance score of ≤ 2
  • Laboratory Values
  • absolute neutrophil count > 500 microliters (mcL)
  • platelet > 50,000 mcL
  • serum aspartate aminotransferase (AST) < 5 x institutional upper limit of normal (IULN)
  • total bilirubin < 3 x IULN
  • serum creatinine < 3 x IULN
  • Pulse oximetry of > 95% on room air.
  • Must have recovered from the toxic effects of all prior chemotherapy

Exclusion Criteria

  • Patients with rapidly progressive disease.
  • Patient is currently receiving any investigational drugs
  • Current cardiomegaly or bilateral pulmonary infiltrates on chest radiograph, pulmonary metastatic lesions are allowed
  • Patients must not have tumor in a location where enlargement could cause airway obstruction
  • Patient is pregnant or lactating
  • History of hypersensitivity reactions to murine protein-containing products.
  • Currently receiving immunosuppressive drugs such as corticosteroids (excluding topical treatment), tacrolimus or cyclosporin
  • Received any tumor vaccines within previous six weeks
  • Known hypersensitivity to rat monoclonal antibodies
  • History of severe allergic reaction to Hepatitis B vaccine, Polio vaccine or Tetanus, Diphtheria, Pertussis vaccine (DTP, Tdap, DT or Td).
  • Allergy to baker's yeast or other components of the vaccines.
  • History of allergy to the antibiotics Neomycin, Streptomycin or Polymyxin B
  • History of coma, long/multiple seizures within 7 days after DTP or Tdap, unless a cause other than the vaccine was indicated.
  • Melanoma involvement of the central nervous system
  • Chemotherapy given within the last 28 days
  • Presence of human anti-mouse antibody (HAMA) prior to enrollment (only patients who have received prior therapy with murine antibodies)

Study Design

Phase
Phase 1
Study Type
Interventional
Allocation
N/A
Intervention Model
Single Group Assignment
Primary Purpose
Treatment
Masking
None (Open Label)

Arm Groups

ArmDescriptionAssigned Intervention
Experimental
tvs-CTL Vaccine
Infusion of activated T-cells generated from a patient's own peripheral blood mononuclear cells.
  • Biological: tvs-CTL Vaccine
    autologous, 14g2a.zeta chimeric receptor transduced, activated T-cells, enriched for vaccine specific cytotoxic T-lymphocytes (tvs-CTL)

Recruiting Locations

KU Cancer Center
Fairway, Kansas 66205
Contact:
Gary Doolittle, MD
913-588-6029
gdoolitt@kumc.edu

More Details

Status
Recruiting
Sponsor
Gary Doolittle

Study Contact

Gary Doolittle, MD
913-588-6029
gdoolitt@kumc.edu

Detailed Description

The rate of progression free survival at one (1) year is < 20% for patients with stage IV metastatic melanoma, despite aggressive cytotoxic chemotherapy regimens and newly approved immunomodulatory and targeted therapy. Immunotherapy seems to hold the most promise for achieving prolonged survival or even cure, therefore,efforts have focused on several different approaches. Such approaches have used tumor vaccination, adoptive transfer of tumor infiltrating lymphocytes, and even monoclonal antibodies, unconjugated or conjugated to cytokines, toxins, or radionucleotides.

The tumor-associated antigen GD2 has been noted on the surface of several tumors, most notably neuroblastoma, but is expressed on melanoma as well. Clinical studies have shown activity of a GD2-specific chimeric T-cell receptor expressed on activated, autologous, T-cells in patients with neuroblastoma. It is the investigators intention to enrich peripheral blood mononuclear cells (PBMC) of patients with stage IV metastatic melanoma with vaccine-specific T-cells through pre-harvest/ phlebotomy vaccination with common, well understood vaccines. The investigators will then modify the T-cells to attack the GD2 antigen. These tumor redirected, vaccine specific, activated T-cells will then be infused into the patient following revaccination with the common vaccines. The Investigators will monitor expansion of the modified T-cells through serial polymerase chain reaction (PCR) assays following vaccination.

The Investigators then intend to re-vaccinate with the selected vaccines one month following infusion and monitor for expansion of the modified T-cells.

Notice

Study information shown on this site is derived from ClinicalTrials.gov (a public registry operated by the National Institutes of Health). The listing of studies provided is not certain to be all studies for which you might be eligible. Furthermore, study eligibility requirements can be difficult to understand and may change over time, so it is wise to speak with your medical care provider and individual research study teams when making decisions related to participation.